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recombinant cho k1 cells  (BPS Bioscience)


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    Structured Review

    BPS Bioscience recombinant cho k1 cells
    Recombinant Cho K1 Cells, supplied by BPS Bioscience, used in various techniques. Bioz Stars score: 91/100, based on 3 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/recombinant+cho+k1+cells/CD22+%2F+Luciferase+-+CHO+Recombinant+Cell+Line/pmc12667665-97-0-12
    Average 91 stars, based on 3 article reviews
    recombinant cho k1 cells - by Bioz Stars, 2026-09
    91/100 stars

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    Related Articles

    Recombinant:

    Article Title: Discovery and characterization of two anti-PD-1 antibodies with a unique binding mechanism to human PD-1
    Article Snippet: Antibody binding was detected by flow cytometry using goat anti-human IgG Fc secondary antibody conjugated to PE (Invitrogen TM Cat. No. 12–4998-82) and measured using IntelliCyt® iQue3 system. .. Recombinant CHO-K1 cells constitutively expressing hPD-L1 (PD-L1/TCR Activator CHO Recombinant cell line, BPS Bioscience Cat. No. 60536) (3.5x10 cells/well) were cultivated on Thaw Medium 3A (BPS Bioscience Cat. No. 60186) overnight at 37°C/5% CO 2 . ..

    Article Title: Chlorin e6-associated photodynamic therapy enhances abscopal antitumor effects via inhibition of PD-1/PD-L1 immune checkpoint
    Article Snippet: .. Recombinant Jurkat T cells constitutively expressing human PD-1 and NFAT reporter genes (#60,535, hPD-1/NFAT Jurkat T cells) and recombinant CHO-K1 cells expressing human PD-L1 and TCR activator were both procured from BPS Bioscience. .. The Jurkat T cells were cultured in RPMI-1640 medium (GE Healthcare Life Sciences, Chicago, IL, USA) supplemented with 10% FBS and antibiotics (100 U/mL penicillin and 100 mg/mL streptomycin).

    Article Title: Sanguisorbae Radix Suppresses Colorectal Tumor Growth Through PD-1/PD-L1 Blockade and Synergistic Effect With Pembrolizumab in a Humanized PD-L1-Expressing Colorectal Cancer Mouse Model
    Article Snippet: The relative chemiluminescence was measured using a SpectraMax L microplate reader (Molecular Devices, San Jose, CA, USA). .. Recombinant Jurkat T cells expressing human PD-1 and NFAT reporter gene (#60535, hPD-1/NFAT Jurkat T cells) and recombinant CHO-K1 cells expressing human PD-L1 and T cell receptor (TCR) activator (#60536, hPD-L1/TCR CHO-K1 cells) were purchased from BPS Bioscience. ..

    Article Title: Evaluation of a Radiolabeled Macrocyclic Peptide as Potential PET Imaging Probe for PD−L1
    Article Snippet: CHO−K1 cells were maintained in Ham's F‐12 medium with 10 % Fetal Bovine Serum (FBS, Gibco) and 1 % Penicillin/Streptomycin (P/S). .. Recombinant CHO−K1 cells stably expressing human PD−L1 (hPD−L1), henceforth referred to as CHO−K1 hPD−L1, were purchased from BPS Bioscience (San Diego, California) and were maintained in F‐12 K medium with 10 % FBS, 1 % P/S, and 2 mg/mL G418. ..

    Expressing:

    Article Title: Discovery and characterization of two anti-PD-1 antibodies with a unique binding mechanism to human PD-1
    Article Snippet: Antibody binding was detected by flow cytometry using goat anti-human IgG Fc secondary antibody conjugated to PE (Invitrogen TM Cat. No. 12–4998-82) and measured using IntelliCyt® iQue3 system. .. Recombinant CHO-K1 cells constitutively expressing hPD-L1 (PD-L1/TCR Activator CHO Recombinant cell line, BPS Bioscience Cat. No. 60536) (3.5x10 cells/well) were cultivated on Thaw Medium 3A (BPS Bioscience Cat. No. 60186) overnight at 37°C/5% CO 2 . ..

    Article Title: Chlorin e6-associated photodynamic therapy enhances abscopal antitumor effects via inhibition of PD-1/PD-L1 immune checkpoint
    Article Snippet: .. Recombinant Jurkat T cells constitutively expressing human PD-1 and NFAT reporter genes (#60,535, hPD-1/NFAT Jurkat T cells) and recombinant CHO-K1 cells expressing human PD-L1 and TCR activator were both procured from BPS Bioscience. .. The Jurkat T cells were cultured in RPMI-1640 medium (GE Healthcare Life Sciences, Chicago, IL, USA) supplemented with 10% FBS and antibiotics (100 U/mL penicillin and 100 mg/mL streptomycin).

    Article Title: Sanguisorbae Radix Suppresses Colorectal Tumor Growth Through PD-1/PD-L1 Blockade and Synergistic Effect With Pembrolizumab in a Humanized PD-L1-Expressing Colorectal Cancer Mouse Model
    Article Snippet: The relative chemiluminescence was measured using a SpectraMax L microplate reader (Molecular Devices, San Jose, CA, USA). .. Recombinant Jurkat T cells expressing human PD-1 and NFAT reporter gene (#60535, hPD-1/NFAT Jurkat T cells) and recombinant CHO-K1 cells expressing human PD-L1 and T cell receptor (TCR) activator (#60536, hPD-L1/TCR CHO-K1 cells) were purchased from BPS Bioscience. ..

    Article Title: Evaluation of a Radiolabeled Macrocyclic Peptide as Potential PET Imaging Probe for PD−L1
    Article Snippet: CHO−K1 cells were maintained in Ham's F‐12 medium with 10 % Fetal Bovine Serum (FBS, Gibco) and 1 % Penicillin/Streptomycin (P/S). .. Recombinant CHO−K1 cells stably expressing human PD−L1 (hPD−L1), henceforth referred to as CHO−K1 hPD−L1, were purchased from BPS Bioscience (San Diego, California) and were maintained in F‐12 K medium with 10 % FBS, 1 % P/S, and 2 mg/mL G418. ..

    Stable Transfection:

    Article Title: Evaluation of a Radiolabeled Macrocyclic Peptide as Potential PET Imaging Probe for PD−L1
    Article Snippet: CHO−K1 cells were maintained in Ham's F‐12 medium with 10 % Fetal Bovine Serum (FBS, Gibco) and 1 % Penicillin/Streptomycin (P/S). .. Recombinant CHO−K1 cells stably expressing human PD−L1 (hPD−L1), henceforth referred to as CHO−K1 hPD−L1, were purchased from BPS Bioscience (San Diego, California) and were maintained in F‐12 K medium with 10 % FBS, 1 % P/S, and 2 mg/mL G418. ..



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    Analytical results of the purified ghC1q variants. A SDS-PAGE of all ghC1q variants under non-reducing and reducing conditions. B Western blot of the ghC1q WT variant stained with 6xHis-tag <t>antibody.</t> <t>CHO-K1</t> host cell line supernatant was used as negative control. WT sample is analysed under non-reducing and reducing conditions and following PNGase F-mediated deglycosylation in reducing conditions
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    Analytical results of the purified ghC1q variants. A SDS-PAGE of all ghC1q variants under non-reducing and reducing conditions. B Western blot of the ghC1q WT variant stained with 6xHis-tag antibody. CHO-K1 host cell line supernatant was used as negative control. WT sample is analysed under non-reducing and reducing conditions and following PNGase F-mediated deglycosylation in reducing conditions

    Journal: BMC Biology

    Article Title: Engineering C1q single-chain globular head variants for enhanced IgM binding

    doi: 10.1186/s12915-026-02570-2

    Figure Lengend Snippet: Analytical results of the purified ghC1q variants. A SDS-PAGE of all ghC1q variants under non-reducing and reducing conditions. B Western blot of the ghC1q WT variant stained with 6xHis-tag antibody. CHO-K1 host cell line supernatant was used as negative control. WT sample is analysed under non-reducing and reducing conditions and following PNGase F-mediated deglycosylation in reducing conditions

    Article Snippet: The corresponding recombinant CHO-K1 cell line (ATCC CCL-61) was developed as described by John et al. [ ] in our laboratories.

    Techniques: Purification, SDS Page, Western Blot, Variant Assay, Staining, Negative Control