Journal: BMC Biology
Article Title: Engineering C1q single-chain globular head variants for enhanced IgM binding
doi: 10.1186/s12915-026-02570-2
Figure Lengend Snippet: Analytical results of the purified ghC1q variants. A SDS-PAGE of all ghC1q variants under non-reducing and reducing conditions. B Western blot of the ghC1q WT variant stained with 6xHis-tag antibody. CHO-K1 host cell line supernatant was used as negative control. WT sample is analysed under non-reducing and reducing conditions and following PNGase F-mediated deglycosylation in reducing conditions
Article Snippet: The corresponding recombinant CHO-K1 cell line (ATCC CCL-61) was developed as described by John et al. [ ] in our laboratories.
Techniques: Purification, SDS Page, Western Blot, Variant Assay, Staining, Negative Control